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ACTICLOT® dPT™ Dilute  Time Test Clinical studies show that a dilute prothrombin time test is an effective LA coagulation assay and can identify LA that are not detected by other tests such as a lupus-sensitive APTT and a dilute Russell’s Viper Venom test.

ACTICLOT® dPT™ Dilute  Time Test  ACTICLOT dPT is a fully integrated dilute prothrombin time test for screening and confirming the presence of phospholipid-dependent LA autoantibodies. The screening protocol utilizes an activator reagent that contains a unique formulation of relipidated recombinant human tissue factor and calcium. The use of recombinant tissue factor in the formulation of the dPT test improves the test’s performance. In the confirmatory protocol, a uniquely formulated phospholipid reagent is used to demonstrate the phospholipid-dependent nature of the LA detected in samples that tested positive in the screening protocol.

Clotting is initiated by activating the extrinsic coagulation pathway with tissue factor in the presence of calcium ions. Tissue Factor binds to Factor VIIa resulting in the activation of Factor IX and Factor X. The subsequent conversion of prothrombin to thrombin by Factor Xa initiates clot formation by cleaving fibrinogen to fibrin. Activation of the tissue factor pathway bypasses the intrinsic (contact) pathway and excludes any interference from deficiencies of Factor XII. REF 824